Genetic Manipulation - Plasmid And Enzyme
Rabu, 17 September 2014
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PLASMID
Plasmid is a double DNA molecule in a ring shape that only exists
in bacteria. In genetic manipulation activity, plasmid functions as vector
(vehicle) used to transfer and duplicate foreign genes.
source: Essential Biology, 2006
Plasmid is a double DNA molecule in a ring shape that only exists
in bacteria. In genetic manipulation activity, plasmid functions as vector
(vehicle) used to transfer and duplicate foreign genes.
Plasmid, a bacteria cell part that has important roles in genetic manipulation activity
ENZYME
The two kinds of enzyme involved in genetic manipulation process are as follows.
- Restriction endonuclease enzyme functions to cut DNA chain, so that it is called biology scissors. This enzyme has abilities to recognize and cut specific nucleotide series in DNA. !
- Ligase enzyme functions to adhere DNA fragments. Look above Picture
Mechanism in the enzyme's work. Nucleotide series known as restriction enzyme, sticky end' motecule of ecombinant DNA
source: Essential Biology, 2006
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BalasHapusBaculovirus,Their double-stranded, circular genomes are 80-180 kbp in size depending on the virus species and the nonoverlapping open reading frames (ORFs) are present in about equal proportions on both DNA strands.
BalasHapus