Plasmid is a double DNA molecule in a ring shape that only exists
in bacteria. In genetic manipulation activity, plasmid functions as vector
(vehicle) used to transfer and duplicate foreign genes.
Plasmid, a bacteria cell part that has important roles in genetic manipulation activity
The two kinds of enzyme involved in genetic manipulation process are as follows.
- Restriction endonuclease enzyme functions to cut DNA chain, so that it is called biology scissors. This enzyme has abilities to recognize and cut specific nucleotide series in DNA. !
- Ligase enzyme functions to adhere DNA fragments. Look above Picture
Mechanism in the enzyme's work. Nucleotide series known as restriction enzyme, sticky end' motecule of ecombinant DNA
source: Essential Biology, 2006