Kamis, 29 Agustus 2013

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The Work Mechanism of Enzyme

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Enzymatic reaction occurs when substrate is available and the active side of enzyme is empty. In this condition, substrate enters the active side' The active site, then, will have shape transformation by enclosing the substrate and form weak chemical bonding in the form of enzyme-substrate complex. Within the active site, substrate will be converted into final form known as product. Next, the product is released from the enzyme bond and the enzyme is free to bind with other substrate.


The followings are two kinds of hypotheses explaining the enzyme mechanism.

Enzyme Mechanism 
a. substrate enters active side of enzyme 
b. it forms enzyme substrate complex, and 
c. product leaves the enzyme 

Lock and key hypothesis. According to the hypothesis, the active side of enzyme has specific and inflexible form. An enzyme only binds with particular substrate. The hypothesis was suggested by Emil Fischer.
 



Lock and key model. Enzyme reaction on substrate produces (a) small size product and (b) big size product.

Induced fit model. The enzyme can change its shape to fit the substrate shape. 

Induced, fit hypothesis. According to the hypothesis, the active side of enzyme is flexible towards the entering of substrate. As the substrate enters the active side, the active side continues changing to fit the substrate shape.

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